Summary Essay Example | Topics and Well Written Essays - 250 words - 143

Outline - Essay Example So as to accomplish the essential point of the exploration, questions, for example, â€Å"what is the middle temperature at which intracellular ice development happens in human oocytes?† â€Å"How promptly does intracellular ice arrangement (IIF) happen in human oocytes?† and â€Å"what is the impact of extracellular seeding on human oocytes?† (Trad et al. 1572-73). These inquiries were replied through an exploratory structure that utilized neglected to-treat and new oocytes with a germinal vesicle and polyspermic eggs. This plan included representation of how IFF first happened at a cooling pace of 120OC/min utilizing a programmable warm magnifying lens stage connected to a video magnifying instrument (Trad et al. 1574). It additionally included execution of extracellular seeding at a cooling pace of 0.2 OC/min so as to diminish the event of IIF and thus increment endurance paces of the freeze-defrosted human oocytes. The plan additionally utilized particular cryoprotectants and diminished the middle temperature in both mouse and human oocytes (Trad et al. 1575). The investigation depicted that IIF happens energetically in human oocytes, and unfavorable IIF can be stayed away from, and endurance rates expanded. The outcomes depicted a 78%, 33% and 0% event of IIF, and the 24 h post-defrost endurance rate was 32%, 56%, and 93% individually (Trad et al. 1576). Trad, Fouad S., Mehmet Toner, and John D. Biggers. Impacts of cryoprotectants and ice-seeding temperature on intracellular freezing and endurance of human oocytes. Human Reproduction 14.6â (1998): 1569-1577.